A Two-dimensional Proteome Map of the Aflatoxigenic Fungus Aspergillus Flavus.

Pechanova, O., Pechan, T., Rodriguez, J. M., Williams, W. P., & Brown, A. E. (2013). A Two-dimensional Proteome Map of the Aflatoxigenic Fungus Aspergillus Flavus. Proteomics. Epub ahead of print, 1-15.

The filamentous fungus Aspergillus flavus is an opportunistic soil-borne pathogen that produces aflatoxins, the most potent naturally occurring carcinogenic compounds known. This work represents the first gel-based profiling analysis of A. flavus proteome and establishes a 2-D proteome map. Using 2-DE and MALDI-TOF-MSMS, we identified 538 mycelial proteins of the aflatoxigenic strain NRRL 3357, the majority of which were functionally annotated as related to various cellular metabolic and biosynthetic processes. Additionally, a few enzymes from the aflatoxin synthesis pathway were also identified.